Welcome to the Kleiner Lab!

Welcome to the Kleiner Lab!

Our lab is part of the Department of Plant & Microbial Biology and the Microbiomes and Complex Microbial Communities Cluster.

Our current projects focus on:

  • Methods for Microbial Community Analyses
    • Development of cutting-edge methods for microbial community analyses focusing on metagenomics and high-resolution mass spectrometry based metaproteomics. A 20 minute overview presentation by Dr. Manuel Kleiner on what we can learn using metaproteomics is available here.
  • Plant-Microbe Interactions
  • Diet-Microbiome Interactions in the Intestinal Tract
    • Harnessing metagenomics, metabolomics, metaproteomics, single cell imaging, and cultivation-based approaches to examine the mechanisms of interaction between intestinal microbiota and external substrates (diet) and host-derived substrates (host compound foraging). This project is supported by the NIH’s MIRA (Maximizing Investigators’ Research Award) grant.
    • Direct linkage of dietary components with metabolizers in the microbiota. This project is sponsored by the Foundation for Food and Agriculture Research (FFAR) and BASF.
    • The fate of dietary protein from different sources and its impact on the intestinal microbiota currently supported through a pilot grant from the Center for Gastrointestinal Biology and Disease (CGIBD).
  • Phage-Microbiome Interactions
    • The discovery and characterization of virotrophs, a hypothesized group of specialized bacteria which are capable of utilizing bacteriophage as a sole nutrient source for growth and replication.
    • Tracking horizontal gene transfer by virus-like particles in microbiomes using transductomics and metaproteomics.
  • Carbon Sequestration
    • The role of microbes in mineral precipitation resulting in exceptional fossil preservation and CO2 sequestration sponsored by the National Science Foundation (NSF).
    • Factors governing energy efficiency of metabolism in free-living and symbiotic bacteria, looking specifically at a novel CO2 fixation pathway.
    • Molecular ecophysiology of microorganisms with two carbon fixation pathways funded by the Binational Science Foundation (BSF).
  • Horizontal Gene Transfer
    • The role of horizontal gene transfer in the metabolic evolution of bacterial symbionts.
  • The saboteur’s tools: mechanisms for host reproductive manipulation by the bacterial arthropod endosymbiont Cardinium hertigii funded by the National Science Foundation (NSF).

Fernanda with part of her switchgrass experiment for looking at plant-microbe interactions using metaproteomics.

Angie cultivating human gut isolates for a study on diet-microbiota interactions.

You can contact us by emailing Dr. Kleiner at manuel_kleiner@ncsu.edu

The Kleiner Lab would like to thank the following funding sources:


Foundation for Food & Agriculture Research